ABSTRACT
Objective To study the apoptosis mechanism of HeLa cells induced by berberine (BR). Methods HeLa cells were treated by different concentrations of BR with different times. CCK-8 assay was used to detect the HeLa cells proliferation activity. Detecting the expression of STAT3 in control HeLa cells and BR treated HeLa cells. HeLa cell cycles were detected by flow cytometry(FCM). The relative expression of STAT3, CYCLIN B1, CDC2 and C-MYC was examined by real-time PCR. The relative expressions of STAT3, CYCLIN B1, CDC2 and C-MYC proteins were examined by Western blot analysis. Results BR can effectively inhibit the proliferation of HeLa cells in vitro, which exhibits a dose-dependent and time-dependent manner. The FCM showed that the the proportion of BR-treated cells in G2/M phase was higher than control group. Real-time PCR results showed that rel-ative expression of STAT3,CYCLIN B1,CDC2 and C-MYC genes in HeLa cells treated with BR was lower than the control group.Western blot results showed that relative expression of STAT3,CYCLIN B1,CDC 2 and C-MYC pro-teins in HeLa cells treated with BR was lower than the control group. Conclusions BR can induce apoptosis of cer-vical cancer by targeting STAT3.
ABSTRACT
Objective To study the apoptosis mechanism of HeLa cells induced by berberine (BR). Methods HeLa cells were treated by different concentrations of BR with different times. CCK-8 assay was used to detect the HeLa cells proliferation activity. Detecting the expression of STAT3 in control HeLa cells and BR treated HeLa cells. HeLa cell cycles were detected by flow cytometry(FCM). The relative expression of STAT3, CYCLIN B1, CDC2 and C-MYC was examined by real-time PCR. The relative expressions of STAT3, CYCLIN B1, CDC2 and C-MYC proteins were examined by Western blot analysis. Results BR can effectively inhibit the proliferation of HeLa cells in vitro, which exhibits a dose-dependent and time-dependent manner. The FCM showed that the the proportion of BR-treated cells in G2/M phase was higher than control group. Real-time PCR results showed that rel-ative expression of STAT3,CYCLIN B1,CDC2 and C-MYC genes in HeLa cells treated with BR was lower than the control group.Western blot results showed that relative expression of STAT3,CYCLIN B1,CDC 2 and C-MYC pro-teins in HeLa cells treated with BR was lower than the control group. Conclusions BR can induce apoptosis of cer-vical cancer by targeting STAT3.
ABSTRACT
<p><b>OBJECTIVE</b>Castrated rats exhibit significant shrinkage of the ventral prostate and apoptosis of prostatic cells, which can be attributed to the reduced blood supply to the prostate. But what causes the blood decrease in the prostate remains unknown. This study aims to explore the molecular mechanism of the changes in the microcirculation of the ventral prostate of rats following castration.</p><p><b>METHODS</b>We randomized 24 male adult rats into 6 groups of equal number, and collected their ventral prostates at 0, 1/2, 1, 2, 3 and 7 d, respectively, after castration. Then we observed the changes of the microvessels under the transmission electron microscope, detected the apoptosis of endothelial cells by TUNEL, and determined the expressions of VEGF, endostatin, angiostatin and angiopoietin-2 by Western blot.</p><p><b>RESULTS</b>The castrated rats showed dramatic changes in the microvessels of the ventral prostate, obvious apoptosis of the endothelial cells, down-regulated expression of VEGF, and up-regulated expressions of endostatin and angiostatin, while angiopoietin-2 remained unchanged.</p><p><b>CONCLUSION</b>The decreased level of VEGF and increased levels of endostatin and angiostatin might underlie the mechanism of the changes in the microcirculation of the ventral prostate of rats following castration.</p>